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Sheep Blood Agar



This is a primary isolation agar used for cultivating a wide range of mastitis organisms from individual cow and bulk tank milk samples. It is available to be made with or without esculin.



This cherry red, translucent agar is prepared from a blood agar base with the addition of 5% sheep erythrocytes and ½ strength esculin. This agar is especially useful for growth of Streptococci. The addition of esculin enhances the capability of differentiating the Streptococci. Certain Streptococci will hydrolyze esculin, causing a mild background blackening for E-strep and a mild greening or no background coloration for suspect Streptococcus agalactiae and most Streptococcus dysgalactiae organisms. All suspect Streptococcus agalactiae organisms would be moved to our special Camp media. UHS blood agar will support good growth of a wide variety of fastidious microorganisms. When reading this agar, anticipate esculin hydrolysis on some coliforms as well.

Refer to table on back for expected culture responses.


Organism Growth Reaction
Strep. agalactiae Excellent Esculin negative
Staph. aureus Excellent Hemolysin detection
Mycoplasma Not Applicable
Strep. dysgalactiae Excellent Esculin negative
Strep. uberis Excellent Esculin positive
E- strep Excellent Esculin positive
Staph. species Excellent
E. Coli Excellent
Klebsiella pneumoniae Excellent
Klebsiella species Excellent
Pseudomonas species Excellent
Pseudomonas aeruginosa Excellent
Pasteurella Excellent
Proteus Excellent
Serratia Excellent
Bacillus Excellent
Yeast Excellent
Mold Excellent
Nocardia Excellent
Prototheca Excellent
Trueperella pyogenes Excellent
C. bovis Excellent

Additional information

Weight .06 lbs
Dimensions 3.5 × 3.5 × .05 in


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