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MP2X Agar



This specialty chromogenic selective agar is used for the cultivation of gram positive bacteria, and to optimize the detection of Staphylococcus aureus from individual cow milk.



This cherry red agar is prepared from a modified Columbia agar base with the addition of 5% washed bovine erythrocytes and contains esculin. The red cells allow for the best possible detection of β-hemolytic zones that will surround the colony on the majority of Staphylococcus aureus strains.  This agar also contains a special chromogen. All Staphylococcus aureus strains will react to the chromogen and produce a green or blue-green colony color. MP2X will also grow other gram positive Staphylococci, Streptococci, and Enterococci bacteria. Some Streptococci will react with the chromogen and appear dark blue, or may hydrolyze esculin, causing a background blackening (including Streptococcus uberis and Enterococcus spp.), but all Streptococci growth will be catalase negative. This selective agar will inhibit the growth of all gram-negative Enterobacteriaceae and Pseudomonas organisms.

All green colonies that show the β-hemolytic zone should be categorized as Staphylococcus aureus. Some nonβ-hemolytic Staphylococcus strains will also produce a green colony and will require a secondary coagulase test. Any nonβ-hemolytic Staphylococcus green colony (either alpha or non-hemolytic) that is coagulase positive should be categorized as Staphylococcus aureus. Any nonβ-hemolytic green Staphylococcus colony that tests coagulase negative should be categorized as Staphylococcus species. There is no need to perform coagulase test on non-green colonies.

Refer to the table on back for the expected culture responses.


Organism Growth Esculin/Beta Chromogen
Strep. agalactiae Excellent Esculin negative Blue
Staph. aureus Excellent Hemolytic (beta) Green
Mycoplasma Not Applicable

Strep. dysgalactiae Excellent

Strep. uberis Excellent Esculin positive Blue
Enterococcus Excellent Esculin positive Blue
Staph. species Excellent

E. Coli Inhibited

Klebsiella pneumoniae Inhibited

Klebsiella species Inhibited

Pseudomonas species Inhibited

Pseudomonas aeruginosa Inhibited

Pasteurella Inhibited

Proteus Inhibited

Serratia Inhibited

Bacillus cereus Delayed

Bacillus licheniformis Inhibited

acillus subtilis Inhibited

Yeast Inhibited

Mold Inhibited

Nocardia Inhibited

Prototheca Inhibited

Trueperella pyogenes Inhibited

C. bovis Inhibited

Additional information

Weight .06 lbs
Dimensions 3.5 × 3.5 × .5 in


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