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Prototheca Agar



This specialty selective agar is used for the cultivation of Prototheca spp. from individual cow milk, bulk tank milk or environmental samples.



This whitish translucent agar is prepared from a purified agar base with the addition of selective agents that inhibit bacterial growth and cultivate Prototheca.  This colorless algae forms small dull white opaque colonies on this agar and are readily observed after 48 hours of incubation.  The agar is highly selective and is formulated to inhibit a wide variety of bacterial organisms, and is advantageous in samples likely to contain mixed populations of microorganisms.  Side by side studies comparing this agar against standard washed cow blood agar with composite cow milk samples, bulk tank samples and pooled string samples have shown substantially increased Prototheca detection.  All suspect colonies that grow on this media should be confirmed microscopically at 200-400X. This confirmation can be easily done with a wet mount or with the use of a stain such as Methylene blue or Gram stain.

Refer to table on back for expected culture responses.


Organism Growth Reaction
Strep. agalactiae Inhibited
Staph. aureus Inhibited
Mycoplasma Inhibited
Strep. dysgalactiae Inhibited
Strep. uberis Inhibited
E- strep Inhibited
Staph. species Inhibited
E. Coli Inhibited
Klebsiella pneumoniae Inhibited
Klebsiella species Inhibited
Pseudomonas species Inhibited
Pseudomonas aeruginosa Inhibited
Pasteurella Inhibited
Proteus Inhibited
Serratia Inhibited
Bacillus Inhibited
Yeast Inhibited
Mold Weak
Nocardia Inhibited
Prototheca Excellent
Trueperella pyogenes Inhibited
C. bovis Inhibited

Additional information

Weight .06 lbs
Dimensions 3.5 × 3.5 × .5 in


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