Description
Approximately 15-25% of mastitis-causing mycoplasma are not identified by the standard Myco. bovis and bovigenitalium targets included with our standard milk and colony PCR kits. This PCR kit will identify the next four most probable candidates; Myco. alkalescens, arginini, californium, and canadense.
This PCR kit may be used on either extracted milk or extracted colonies. It is intended to be run on samples that were already confirmed to be positive by another PCR kit.
| Kit Type | DNA qPCR |
| Reactions Provided | 100 (20 µL) |
| Positive Control | gDNA, included |
| Negative Control | Included |
| Composition | Five assays on Five dyes |
| Associated Extraction Kit | EXT-001, EXT-002, EXT-004 |
| Validated Instruments | Bio-Rad CFX96, CFX96 Deep Well, Precigenome Fast 16-6 |
| Dyes Used | FAM, SUN, Cal Fluor Red 610, Atto 647n, Quasar 705 |
| Targets | Mycoplasma spp. (FAM), M. calif. (SUN), M. alkalescens (CFR610), M. arginini (Atto 647n), M. canadense (Q705). |
| Thermal Cycling Time | ~57 minutes |
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