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PCR-003B: Mycoplasma Validation and Speciation Kit



This PCR kit is designed to verify mycoplasma colonies grown on agar. It also identifies the common species Mycoplasma bovis and bovigenitalium.


Mycoplasma culture is a popular and well-established method of detecting mastitis-causing mycoplasma in dairy cattle. However, a significant problem is false positive results caused by Acholeplasma, a non-pathogenic look-alike organism. The PCR-003B kit will determine if colonies tested are Mycoplasma or Acholeplasma, allowing confident herd management. Furthermore, the two most common species Myco. bovis and bovigenitalium are identified. Not all mycoplasma species are equally pathogenic or demand the same management strategies, and this information may be useful in determining the urgency of intervention strategies.

The PCR-003B kit is designed to be used alongside the EXT-002 Colony Extraction Kit.


Kit Type DNA qPCR
Reactions Provided 100 (20 µL)
Positive Control gDNA, included
Negative Control Included
Composition Four assays on four dyes
Associated Extraction Kit EXT-002
Validated Instruments Bio-Rad CFX96, CFX96 Deep Well, Precigenome Fast 16-6
Dyes Used FAM, SUN, ROX, Atto 647n
Targets Mycoplasma spp. (FAM), Acholeplasma spp. (SUN), M. bovigenitalium (ROX), M. bovis (Atto 647n).
Thermal Cycling Time ~53 minutes


Additional information

Weight .06 lbs
Dimensions 3.5 × 3.5 × .05 in


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